Riboseq-flow: A streamlined, reliable pipeline for ribosome profiling data analysis and QC

Ribosome profiling is a cutting-edge method that provides a detailed view of protein synthesis across the entire set of RNA molecules within cells. To ensure the reliability of such studies, high-quality data and the ability to replicate analyses are crucial. To address this, we present riboseq-flow, a new tool built with Nextflow DSL2, tailored for analysing data from ribosome profiling experiments. This pipeline stands out for its ease of use, flexibility, and commitment to high reproducibility standards. It's designed to handle multiple samples simultaneously, ensuring efficient analysis for large-scale studies. Moreover, riboseq-flow automatically generates detailed reports and visual representations to assess the data quality, enhancing researchers' understanding of their experiments and guiding future decisions. We hope that our modules and workflow will be useful assets for integration with complementary pipelines (e.g. nf-core/riboseq) and community endeavours in developing cutting-edge ribo-seq analysis pipelines.